PIWIL1 mRNA expression in the normal lung epithelial cell line (CRL-9482) and in two NSCLC cell lines (A549 and NCI-H1299) was investigated by RT-PCR analysis. The RT-PCR analysis shows that the PIWIL1 mRNA levels are significantly higher in the A549 and NCI-H1299 compared to that in CRL-9482. The RT-PCR analysis was carried out using the 2(−Delta Delta C (T)) Method..
Reeves et al. BMC Research Notes 2012 5:239 doi:10.1186/1756-0500-5-239