The leukotoxin-neutralizing compounds bind to the bacteria-associated toxin upon neutralization. LDH-release from macrophages (PMA-treated THP-1 cells) exposed to highly leukotoxic A. actinomycetemcomitans (strain HK1519) 10 bacteria/cell for 60 min. The macrophages were exposed to native bacteria, bacteria pre-treated with Guava extract (0.1%) or to bacteria in the presence of Guava (0.1%). Mean ± SD of 3 experiments is shown and significant leukotoxin neutralization is indicated (*).
Kwamin et al. BMC Research Notes 2012 5:203 doi:10.1186/1756-0500-5-203