Denaturing gel electrophoresis of RNA isolated from different plant tissues using IHBT protocol. Name of plant tissues along with RNA yield (μg/100 mg tissue) is written above each panel; A260/280 ratio is mentioned at the bottom of the panel. The quality was assessed by electrophoresing an equal volume of RNA (2 μl) on formaldehyde-agarose denaturing gel to observe integrity of 28 S and 18 S rRNA bands, and by confirming the amenability of isolated RNA to reverse transcription-polymerase chain reaction based amplification of 26 S rRNA. Tea (Camellia sinensis, Family: Theaceae), potato (Solanum tuberosum, Family: Solanaceae), chili pepper (Capsicum annum, Family: Solanaceae), picrorhiza (Picrorhiza kurrooa, Family: Scrophulariaceae), stevia (Stevia rebaudiana, Family: Asteraceae), aromatic turmeric (Curcuma aromatica, Family: Zingiberaceae), and lantana (Lantana camara, Family: Verbenaceae) is rich in flavanoids, polysaccharides, capsaicinoids, picrosides, steviosides, curcuminoids, and triterpenoids, respectively. Arabidopsis (Arabidopsis thaliana, Family: Brassicaceae) is a model plant, whereas the knowledge on the nature of secondary metabolites in caragana (Caragana jubata, Family: Fabaceae), Himalayan cinquefoil (Potentilla atrosanguinea, Family: Rosaceae), cotton (Gossypium hirsutum, Family: Malvaceae), toona (Toona sinensis, Family: Meliaceae), and white clover (Trifolium repens, Family: Fabaceae) was not available.
Ghawana et al. BMC Research Notes 2011 4:85 doi:10.1186/1756-0500-4-85