Table 1

Classification of wild boar sera through five freeze-thaw cycles.

Clean

Haemolysed


Freeze/thaw cycle

1

2

3

4

5

1

2

3

4

5


Sample number

1

1

1

1

1

1

1

1

1

1

1

2

1

1

1

1

1

1

1

1

1

1

3

0

0

0

0

0

0

0

0

0

2

4

0

0

0

0

2

0

0

0

0

2

5

0

0

0

0

0

0

0

0

0

0

6

1

1

1

1

1

1

1

1

1

1

7

1

1

1

1

1

1

1

1

1

1

8

0

0

0

0

0

0

0

2

0

2

9

1

1

1

1

1

0

0

0

2

1

10

1

1

1

1

1

1

1

1

1

1

11

0

0

0

0

0

1

1

1

1

1

12

1

1

1

1

1

1

1

1

1

1

13

0

2

0

0

0

2

2

0

0

0

14

1

1

1

1

1

1

1

1

1

1

15

0

0

0

0

0

0

0

0

0

2

16

1

1

1

1

1

1

1

1

1

1

17

0

0

0

0

0

0

0

0

2

2

18

0

0

0

0

2

19

0

0

0

0

0

20

1

1

1

1

1


N° positive samples

10

10

10

10

10

9

9

9

9

10

N° negative samples

10

9

10

10

8

7

7

7

6

2

N° doubtful samples

0

1

0

0

2

1

1

1

2

5

N° changing state

1

0

0

2

0

2

3

7

% changing state

5%

0%

0%

10%

0%

12%

18%

41%


Classification of the 20 wild boar sera samples (20 clean; 17 haemolysed) after 1 to 5 freeze-thaw cycles into positive (1), negative (0) or doubtful (2) according to a commercial ELISA for the detection of serum immunoglobulin G antibodies against Aujeszky's disease virus.

Boadella and Gortázar BMC Research Notes 2011 4:498   doi:10.1186/1756-0500-4-498

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