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Open Access Highly Accessed Short Report

Invasion of Ureaplasma diversum in bovine spermatozoids

Melissa Buzinhani, Maurício Yamaguti, Rosângela C Oliveira, Beatriz A Cortez, Lucas Miranda Marques, Gláucia M Machado-Santelli, Mayra EO Assumpção and Jorge Timenetsky*

Author Affiliations

Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brasil

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BMC Research Notes 2011, 4:455  doi:10.1186/1756-0500-4-455

Published: 27 October 2011

Abstract

Background

Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze in vitro interaction of U. diversum isolates and ATCC strains with bovine spermatozoids. The interactions were observed by confocal microscopy and the gentamycin internalization assay.

Findings

U. diversum were able to adhere to and invade spermatozoids after 30 min of infection. The gentamicin resistance assay confirmed the intracellularity and survival of U. diversum in bovine spermatozoids.

Conclusions

The intracellular nature of bovine ureaplasma identifies a new difficulty to control the reproductive of these animals.

Keywords:
Ureaplasma diversum; bovine spermatozoid; invasion; confocal microscopy