Open Access Highly Accessed Technical Note

RNA isolation for transcriptomics of human and mouse small skin biopsies

Oskar Bruning1, Wendy Rodenburg2, Teodora Radonic3, Aeilko H Zwinderman3, Annemieke de Vries2, Timo M Breit1* and Mark de Jong1

Author Affiliations

1 MicroArray Department & Integrative Bioinformatics Unit (MAD-IBU), Swammerdam Institute for Life Sciences (SILS); Faculty of Science (FNWI), University of Amsterdam (UvA), Science Park 904, 1098 XH Amsterdam, The Netherlands

2 Laboratory for Health Protection Research (GBO), National Institute of Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, 3721 MA Bilthoven, The Netherlands

3 Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Academic Medical Center (AMC) Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands

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BMC Research Notes 2011, 4:438  doi:10.1186/1756-0500-4-438

Published: 24 October 2011

Additional files

Additional file 1:

RNA isolation protocol. Step-by-step protocol describing the isolation of RNA from skin biopsies.

Format: PDF Size: 41KB Download file

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Additional file 2:

Human biopsies results. Table showing the quality measure results for individual RNA samples isolated from human skin biopsies.

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Additional file 3:

Scatterplots Human biopsies. Same figure as Figures 1B-E, now showing extreme outliers.

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Additional file 4:

Mouse biopsies results. Table showing the quality measure results for individual RNA samples isolated from mouse skin biopsies.

Format: XLS Size: 24KB Download file

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Additional file 5:

Mouse biopsy sampling. Overview of resulting holes on mouse skin after sampling with different punch diameters.

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Additional file 6:

Comparison to the gold standard methods. Table showing the quality measure results of this study compared to the gold standard methods for RNA isolation from skin biopsies.

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