Figure 4.

EcoTILLING screening - Figure shows images related to the EcoTILLING screening conducted on 68 samples amplified with the primer pair for Region 7. EcoTILLING analysis did not report the presence of some mutated samples (1) which were identified through HRM (2) and confirmed by sequencing (3). (4) EcoTILLING experiments done by varying the digestion time or the number of samples per pool did not change the results. a) Digestion times for pools with CEL I or ENDO-1. Lane 1: 15'; lane 2: 20'; lane3: 30'. Images of a single detection channel. b) Proportion variations between mutated samples and wild type.