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Open Access Highly Accessed Technical Note

Simultaneous DNA and RNA isolation from brain punches for epigenetics

Marc Bettscheider, Chris Murgatroyd* and Dietmar Spengler

Author Affiliations

Department of Molecular Neuroendocrinology, Max-Planck-Institute of Psychiatry, Kraepelinstr. 2-10, 80804 Munich, Germany

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BMC Research Notes 2011, 4:314  doi:10.1186/1756-0500-4-314

Published: 30 August 2011

Abstract

Background

Epigenetic modifications such as DNA methylation play an important role for gene expression and are regulated by developmental and environmental signals. DNA methylation typically occurs in a highly tissue- and cell-specific manner. This raises a severe challenge when studying discrete, small regions of the brain where cellular heterogeneity is high and tissue quantity limited. Because gene expression and methylation are often tightly linked it appears of interest to compare both parameters in the same sample.

Findings

We present a refined method for the simultaneous extraction of DNA for bisulfite sequencing and RNA for expression analysis from small mouse brain tissue punches. This method can also be easily adapted for other small tissues or cell populations.

Conclusions

The method described herein results in DNA and RNA of a quantity and quality permitting highly reliable bisulfite analysis and quantitative RT-PCR measurements, respectively.