Table 1 |
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|
Primer pairs used to amplify the 3 target genes tilled in both EMS-mutant populations |
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|
Gene |
Forward (*LabelledIRD700) |
Reverse (*LabelledIRD800) |
|
|
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|
Cm-eIF4E 1st amplicon |
GACTCAAACGCCTAACAGAAAATC |
CTATCTCACCAAGTTTCCTAAATT |
|
|
||
|
*GAGGGCGGTGCCATTCTTCTTCGG |
*TCCCTAAATCGAACCAAGAAACGCC |
|
|
|
||
|
Cm-eIF4E 2nd amplicon |
TTGTTTCGTGTTGACATGTCCATC |
AGCATGCATTTCACCTCATTGGCT |
|
|
||
|
*TGCTTGGCTGTTAATTTATCTCTGC |
*GTCAAGTACAGAACAAGAATCTGAG |
|
|
|
||
|
Cm-eIF(iso)4E |
GATCGATAATTTCCCTTTTC |
TGACAGGCTTAAGCACTATG |
|
|
||
|
*ACCCCAATTCATTCTAGGG |
*TGTGCAAGCGCAACAAGGTAC |
|
|
|
||
|
Cm-PDS |
CAATGGGATGCTTAGATCAAC |
TCCTTCCAAAATTACCCTAG |
|
|
||
|
*GGATTTCTCGGAGTGACTCGG |
*GCTCTCGAGCAACTAGCACT |
|
|
|
||
|
Nested PCR was used in all cases with labeled primers in the second PCR. |
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|
González et al. BMC Research Notes 2011 4:289 doi:10.1186/1756-0500-4-289 |
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