Open Access Short Report

Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice

Marcilia P Costa12, John A McCulloch3, Síntia S Almeida1, Fernanda A Dorella1, Cristina T Fonseca4, Diana M Oliveira2, Maria FS Teixeira2, Ewa Laskowska5, Barbara Lipinska5, Roberto Meyer6, Ricardo W Portela6, Sérgio C Oliveira1, Anderson Miyoshi1 and Vasco Azevedo1*

Author Affiliations

1 Departamento de Biologia Geral. Instituto de Ciências Biológicas. Universidade Federal de Minas Gerais. Av. Antonio Carlos, 6627 - Pampulha, CP 486, CEP 31.270-901. Belo Horizonte- MG, Brazil

2 Faculdade de Medicina Veterinária, Núcleo de Genômica e Bioinformática. Universidade Estadual do Ceará. Av. Paranjana, 1700 - Itaperi, CEP 60.740-000. Fortaleza-CE, Brazil

3 Departamento de Genética. Instituto de Ciências Biológicas. Universidade Federal do Pará, R. Augusto Corrêa, 01 - Guamá, CP 8607, CEP 66.075-900. Belém-PA, Brazil

4 Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou, Av. Augusto Lima, 1715 - Barro Preto, CEP 30.190-002. Belo Horizonte-MG, Brazil

5 Department of Biochemistry. University of Gdansk, ul. Bażyńskiego 1a, Postal Code 80-952. Gdańsk, Poland

6 Departamento de Ciências da Biointeração. Instituto de Ciências da Saúde. Universidade Federal da Bahia, Av. Reitor Miguel Calmon, s/n - Vale do Canela, CEP 40110100. Salvador-BA, Brazil

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BMC Research Notes 2011, 4:243  doi:10.1186/1756-0500-4-243

Published: 20 July 2011



Heat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the hsp60-hsp10 bicistronic operon of the animal pathogen Corynebacterium pseudotuberculosis, a Gram-positive bacterium of the class Actinobacteria, which causes caseous lymphadenitis (CLA) in small ruminants.


To construct the DNA vaccine, the hsp60 gene of C. pseudotuberculosis was cloned in a mammalian expression vector. BALB/c mice were immunized by intramuscular injection with the recombinant plasmid (pVAX1/hsp60).


This vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production. However, immunization with this DNA vaccine did not confer protective immunity.