A screen for peptide agonists of the G-CSF receptor
SYGNIS Bioscience, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany
BMC Research Notes 2011, 4:194 doi:10.1186/1756-0500-4-194Published: 15 June 2011
Granulocyte-colony stimulating factor (G-CSF) is one of the most important pharmacologically used proteins. Potential uses beyond the stimulation of neutrophilic granulocytes are the treatment of CNS disorders. Disadvantages of the G-CSF protein as a drug are its moderate plasma half-life time and considerable production costs. We therefore conducted a screen for peptide agonists derived from the sequence of human G-CSF.
Despite of the high sensitivity of our screening system we could not detect any positive hits in a single peptide approach. In a multiplex approach using a permutation of any combination of 10 different peptides we could also not detect a positive block.
We conclude that larger coherent parts of the protein or dimerising peptides may be needed to achieve activation of the receptor.