Table 2

Recommendation for the use of different methods for RNA isolation from cartilage/chondrocytes.

METHOD

MATERIAL


chondrocytes

comments

cartilage

comments


Trizol®

++

solid RNA quality

-

RNA degradation

high RNA yield

RNeasy™

+++

high RNA quality

-

RNA degradation

solid RNA yield

Trizol®/RNeasy™

+

cost-benefit ratio (-)

+/++

solid RNA quality

solid RNA quality

(difficulties with human samples)

solid RNA yield

solid RNA yield

RNAqueous

+

cost-benefit ratio (-)

+++

high RNA quality

solid RNA quality

solid RNA yield

solid RNA yield


The results/criteria for recommendation are given as follows: - fail; + passing; ++ good; +++ very good.

Ruettger et al. BMC Research Notes 2010 3:7   doi:10.1186/1756-0500-3-7

Open Data