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Open Access Highly Accessed Technical Note

Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis in vivo

Anke Ruettger12*, Steffi Neumann23, Bernd Wiederanders4 and René Huber5

Author Affiliations

1 Institute of Molecular Pathogenesis (IMP), Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Jena, Germany

2 Research Unit at the Waldkrankenhaus "Rudolf Elle", Department of Orthopaedics, University Hospital Jena, Eisenberg, Germany

3 Institute of Diagnostic and Interventional Radiology, University Hospital Jena, Jena, Germany

4 Institute of Biochemistry I, University Hospital Jena, Jena, Germany

5 Institute of Clinical Chemistry, Hannover Medical School, Hannover, Germany

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BMC Research Notes 2010, 3:7  doi:10.1186/1756-0500-3-7

Published: 18 January 2010

Additional files

Additional file 1:

Comparative analysis of current methods for RNA isolation from cartilage/chondrocytes. This table provides RNA quality control parameters (detected with the NanoDrop) and parameters of cell yields after chondrocyte extraction from cartilage.

Format: PDF Size: 26KB Download file

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Open Data

Additional file 2:

Protocol 1 - RNA isolation from cartilage using RNAqueous Midi™ kit. This data file provides a complete protocol for using the RNAqueous Midi™ kit. It enables the reader to start immediately with RNA isolation. This protocol is the best one for RNA isolation from human cartilage samples.

Format: PDF Size: 57KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Protocol 2 - Combined method for RNA isolation from cartilage. This data file provides a complete protocol for using the combined method. It enables the reader to start immediately with RNA isolation. This protocol is acceptable for RNA isolation from bovine cartilage samples.

Format: PDF Size: 66KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4:

Primers, product length, and specific amplification conditions for (q)RT-PCR. This table provides additional information about primers and amplification conditions for qRT-PCR and RT-PCR.

Format: PDF Size: 20KB Download file

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Open Data

Additional file 5:

Characterization of special parameters during RNA isolation from bovine articular cartilage. In this figure we compare special parameters during RNA isolation based on Agilent analysis.

Format: PDF Size: 47KB Download file

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Open Data

Additional file 6:

Quality control of total RNA from human cartilage explants from one typical donor using RT-PCR. In this figure, we present the results of an typical gel electrophoresis image after RT-PCR.

Format: PDF Size: 112KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data