Extension of sequence from clone 143-2-1 in the 5' (A) and 3' (B) directions. First round TVL-PCR products (top) were generated from templates prepared using HindIII (H), EcoRI (E), or BamHI (B) digestion of genomic DNA. First round products were used as templates to generate second round products (bottom). Boxes (bottom gels) indicate the locations of the second round TVL-PCR products that proved to be the desired products.
Orcheski and Davis BMC Research Notes 2010 3:61 doi:10.1186/1756-0500-3-61