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Morphological analysis of the sheathed flagellum of Brucella melitensis

Jonathan Ferooz12 and Jean-Jacques Letesson1*

Author Affiliations

1 Unité de Recherche en Biologie Moléculaire (URBM), Facultés Universitaires Notre-Dame de la Paix (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium

2 GlaxoSmithKline Biologicals, 20 Avenue Fleming, B-1300 Wavre, Belgium

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BMC Research Notes 2010, 3:333  doi:10.1186/1756-0500-3-333

Published: 9 December 2010

Abstract

Background

It was recently shown that B. melitensis is flagellated. However, the flagellar structure remains poorly described.

Findings

We analyzed the structure of the polar sheathed flagellum of B. melitensis by TEM analysis and demonstrated that the Ryu staining is a good method to quickly visualize the flagellum by optical microscopy. The TEM analysis demonstrated that an extension of the outer membrane surrounds a filament ending by a club-like structure. The ΔftcR, ΔfliF, ΔflgE and ΔfliC flagellar mutants still produce an empty sheath.

Conclusions

Our results demonstrate that the flagellum of B. melitensis has the characteristics of the sheathed flagella. Our results also suggest that the flagellar sheath production is not directly linked to the flagellar structure assembly and is not regulated by the FtcR master regulator.