Table 1

Designations and sequences of primers used in RT-PCR

Name

Sequence (5'-3')

Ghrl

Exon

Ta (°C)

PCR Cycles

RACEout-F

AATTCGTCACTCCGTGAATCAG

N/A

RACEout-R

CAGAGCATGCTGAGTAGCAG

1

62

25

RACEin-F

GTCACTCCGTGAATCAGATCG

N/A

RACEin-R

CAGCAAACTGCAGATGGTG

1

61

35

Ext1-F

AAGGCACATAACATGGAGATGAAG

1*

Ex1-R

CTTGGTGGTGAGGACAGATGAC

1

60

40

Ex4-R

GCCTGTCCGTGGTTACTTGT

4

58

40

Gapdh-F

ACCTGCCAAGTATGATGACATCA

N/A

Gapdh-R

GGTCCTCAGTGTAGCCCAAGAT

N/A

60

40

Annealing temperatures (Ta) of oligonucleotide primers employed in RT-PCR are shown. The location of oligonucleotide primers spanning ghrelin exons are listed, while oligonucleotides spanning synthetic sequences (adapters and linkers) or genes other than Ghrl are denoted as N/A (not applicable). The novel extended exon 1 is denoted as 1*. All primers were synthesised by Proligo (Armidale, Australia).

Seim et al. BMC Research Notes 2009 2:85   doi:10.1186/1756-0500-2-85