Figure 5.

Real-time RT-PCR demonstrating tissue-specific expression of murine ghrelin transcripts with an extended exon 1. Relative expression of extended exon 1 of Ghrl in a wide range of mouse tissues (TissueScan Mouse Normal Tissue qPCR array). Calculations of Ghrl expression levels were performed using the standard curve method (correlating the threshold cycle number (C_T values) and copy numbers of Ghrl) and normalised to the expression of Gapdh.