Table 1

Expression values of 6 reference genes using qPCR in purified versus non-purified pre-amplified samples

UBC

GAPDH

RPL13A

YWHAZ

SDHA

HPRT1

mean dCq

2^dCq


sample 1

mean Cq NP1 (n = 2)

25.47

23.73

19.05

22.31

29.82

25.71

mean Cq P1 (n = 2)

21.54

22.19

15.87

18.29

25.94

22.03

dCq

3.93

1.54

3.18

4.02

3.88

3.68

3.37

10.34


sample 2

mean Cq NP2 (n = 2)

23.71

23.03

18.12

21.81

30.14

24.86

mean Cq P2 (n = 2)

20.95

21.54

15.63

18.53

28.14

21.89

dCq

2.77

1.49

2.49

3.28

2.01

2.97

2.5

5.65


sample 3

mean Cq NP3 (n = 2)

24.22

23.03

18.63

20.94

30.17

25.82

mean Cq P3 (n = 2)

21.1

21.75

16.04

17.66

28.67

22.72

dCq

3.12

1.29

2.6

3.28

1.5

3.1

2.48

5.57


sample 4

mean Cq NP4 (n = 2)

22.96

23.08

18.54

21.74

30.66

25.62

mean Cq P4 (n = 2)

19.69

21.89

16.07

18.63

28.83

22.48

dCq

3.27

1.19

2.48

3.11

1.83

3.14

2.5

5.66


sample 5

mean Cq NP5 (n = 2)

23.21

22.72

17.96

20.84

28.17

25.37

mean Cq P5 (n = 2)

20.64

21.22

15.6

17.83

25.73

22.11

dCq

2.57

1.51

2.36

3.01

2.44

3.26

2.52

5.74


sample 6

mean Cq NP6 (n = 2)

24.62

23.48

18.56

22.22

28.84

25.71

mean Cq P6 (n = 2)

21.52

21.87

15.91

19.03

26.97

22.67

dCq

3.1

1.61

2.65

3.19

1.88

3.04

2.58

5.96


average

6.31 (95% CI: 4.89 - 8.14)


Cq: quantification cycle

dCq: difference in Cq or delta-Cq

NP: non-purified after pre-amplification

P: purified after pre-amplification

2^dCq designates the pre-amplifiable target ratio (purified/non-purified products)

Vermeulen et al. BMC Research Notes 2009 2:235   doi:10.1186/1756-0500-2-235

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