Table 1

Essential E. coli genes with high ROL values are also essential in Acinetobacter.

K12 gene

Gene function

ROL γ-β

ROL α

ROL B-M

Essential


asd

ASA dehydrogenase

3.07

n.a.

n.a.

DB

can

carbonic anhydrase

4.07

11.67

n.a.

-

degS

serine endoprotease

8.63

9.54

7.77

NON

dnaC

DNA biosynthesis protein

62.30

n.a.

94.06

-

fabA

HD thioester dehydrase

2.78

2.42

n.a.

-

fabB

3-oxoacyl-[acp] synthase

3.55

2.75

n.a.

DB

fbaA

FBP aldolase

3.21

n.a.

n.a.

-

fldA

flavodoxin 1

4.73

22.92

16.86

-

ftsE

transporter subunit

3.05

2.48

8.79

-

ftsK

chromosome partitioning

7.20

2.55

10.41

ESS

ftsL

cell division protein

4.59

n.a.

n.a.

-

ftsN

cell division protein

12.64

n.a.

n.a.

-

ftsX

transporter subunit

2.87

13.32

9.10

-

hemD

uroporphyrinogen synthase

2.75

n.a.

n.a.

DB

hemG

protoporphyrin oxidase

8.47

n.a.

n.a.

-

holB

DNA ROL III subunit

5.08

19.02

32.24

-

lolB

chaperone for lipoproteins

2.67

n.a.

n.a.

ESS

lolE

lipoprotein transporter

7.92

5.89

55.12

-

mreD

cell wall structural complex

3.53

n.a.

n.a.

-

mukB

chromosome partitioning

13.42

n.a.

94.78

-

mukE

chromosome partitioning

7.41

n.a.

n.a.

-

mukF

chromosome partitioning

7.16

n.a.

n.a.

-

nrdA

RDP reductase subunit

4.91

n.a.

n.a.

-

nrdB

RDP reductase subunit

3.03

5.75

n.a.

ESS

plsB

O-acyltransferase

5.09

n.a.

n.a.

ESS

plsC

acyltransferase

4.06

5.79

32.81

-

psd

decarboxylase

3.28

36.74

13.10

ESS

pssA

phosphatidylserine synthase

7.12

n.a.

n.a.

-

rlpB

minor lipoprotein

8.13

n.a.

n.a.

-

secM

regulator of secA

10.80

n.a.

n.a.

-

yejM

hydrolase, inner membrane

6.76

n.a.

n.a.

-

yrbK

conserved protein

8.94

n.a.

n.a.

-

yrfF

inner membrane protein

7.93

n.a.

n.a.

-

zipA

cell division protein

3.33

n.a.

n.a.

-


Only those E. coli K12 genes with high ROL values in γ-β-proteobacteria, α-proteobacteria, and Bacilli-Mollicutes (ROL values greater than 2.4 in all three groups (Fig. 1)) are shown here. Of these 34 genes, nine have an orthologue in Acinetobacter, and eight of these have an essential function in Acinetobacter. Many of the genes lost at high rates from γ-β-proteobacteria have orthologues in fewer than 10% of the α-proteobacteria or Bacilli-Mollicutes; these are indicated by n.a. ESS, genes for which no deletion mutants were isolated in Acinetobacter; DB, genes in which only "double band" mutants containing chromosomal duplications were isolated in Acinetobacter (and which are thus likely to be essential); NON, genes for which deletion mutants were isolated in Acinetobacter, and which are thus considered to be non-essential; [9]. '-', genes without an orthologue in Acinetobacter.

Silander and Ackermann BMC Research Notes 2009 2:2   doi:10.1186/1756-0500-2-2

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