Demonstration of sequence extension using BEAP. Example of a contig created in the Angus dwarfism locus by BEAP. The contig is aligned, using the align two sequences tool at NCBI , to the first BLAST match to the template sequence retrieved from NCBI. The alignment shows both successful retrieval of target sequence in cattle and nearly tripling in the size of the template for primer design and lab analysis (883 bps for individual sequence vs. 2237 bps for constructed contig). The score, expect, identity and gap statistics show the contig was used in its entirety, with no gaps and 100% sequence match.
Koltes et al. BMC Research Notes 2009 2:11 doi:10.1186/1756-0500-2-11