Table 2

Genotype call and concordance percentage rates for DNA samples extracted from FTA cards, by sample type.

Standard

Blood FTA

Blood FTA Elute

Nasal Swab FTA Elute

Call Rate %

98.86

99.04 (0.07)

98.44 (0.44)

95.78 (0.25)

Concordance Rate %

99.006

99.817 (0.40)

99.786 (0.40)

99.573 (0.43)

Alternative Homozygous Rate %

0.006

0.000 (0.40)

0.000 (0.40)

0.002 (0.43)

Homozygous vs. Heterozygous Rate %

0.988

0.183 (0.40)

0.214 (0.40)

0.425 (0.43)

Two sample t-tests assuming equal within-treatment variances, the number in parenthesis is the P-value corresponding to the comparison of that sample to the standard. Two samples from each FTA type were compared to 7,737 samples extracted by PCI for call rate and to 35 samples extracted by PCI for which dual aliquots were genotyped on the Bovine SNP50 BeadChip for concordance, alternative homozygous, and homozygous vs. heterozygous rate.

McClure et al. BMC Research Notes 2009 2:107   doi:10.1186/1756-0500-2-107