Expression of 5S rDNA in the oocytes of water frogs
Department of Animal Physiology and Development, Institute of Experimental Biology, Adam Mickiewicz University, Umultowska Str. 89, 61-614 Poznañ, Poland
BMC Research Notes 2009, 2:10 doi:10.1186/1756-0500-2-10Published: 19 January 2009
We report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR. This kind of research had never been performed before.
5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes. The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.
We suggest the possibility of using 5S rDNA as the internal control gene, in the studies of relative mRNA quantitative assays in water frog oocytes, because of its characteristic specific expression pattern in the Rana lessonae, Rana ridibunda and Rana esculenta oocytes.