Table 3 |
||||
|
Number of validated genes of two serial dilutions of 3 cDNA samples before and after their preamplification. |
||||
|
Initial cDNA |
||||
|
|
||||
|
25 ng of preamplified cDNA |
||||
|
|
||||
|
Sample number |
N° validated genes (CT ≤ 31) |
N° validated genes with ΔΔCT outside ± 1.5 |
r |
|
|
|
||||
|
NPA samples |
PA samples |
|||
|
|
||||
|
2 |
39 |
46 |
4 |
0.978 |
|
11 |
41 |
46 |
3 |
0.956 |
|
22 |
40 |
45 |
1 |
0.968 |
|
|
||||
|
ng cDNA/port |
500 |
15000 |
||
|
|
||||
|
Dilution 1/20 of the initial cDNA |
||||
|
|
||||
|
1.25 ng of preamplified cDNA |
||||
|
|
||||
|
Sample number |
N° validated genes (CT ≤ 31) |
N° validated genes with ΔΔCT outside ± 1.5 |
r |
|
|
|
||||
|
NPA samples |
PA samples |
|||
|
|
||||
|
2 |
14 |
38 |
6 |
0.972 |
|
11 |
22 |
40 |
2 |
0.967 |
|
22 |
6 |
39 |
5 |
0.988 |
|
|
||||
|
ng cDNA/port |
25 |
750 |
||
|
|
||||
|
Dilution 1/400 of the initial cDNA |
||||
|
|
||||
|
0.06 ng of preamplified cDNA |
||||
|
|
||||
|
Sample number |
N° validated genes (CT ≤ 31) |
N° validated genes with ΔΔCT outside ± 1.5 |
r |
|
|
|
||||
|
NPA samples |
PA samples |
|||
|
|
||||
|
2 |
3 |
17 |
0 |
0.962 |
|
11 |
2 |
27 |
3 |
0.883 |
|
22 |
3 |
20 |
2 |
0.932 |
|
|
||||
|
ng cDNA/port |
1.25 |
37.5 |
||
|
|
||||
|
Legend/key: The number of each sample corresponds to the "sample number" indicated in Table 2. CT, Cycle Threshold; NPA, Non-Preamplified; PA, Preamplified. |
||||
|
Mengual et al. BMC Research Notes 2008 1:21 doi:10.1186/1756-0500-1-21 |
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