Figure 1.

Workflow for the entire preamplification process. Detailed steps are described in the Material and Methods section. Briefly, to increase the quantity of the specific cDNA targets for gene expression analysis using TaqMan methodology, cDNA from the reverse transcription is mixed with pooled TaqMan Gene Expression Assays and with TaqMan PreAmp MasterMix. After the multiplex preamplification of desired cDNA targets, quantitative real time PCR amplification of preamplified target cDNAs, using sequence-specific primers and TaqMan probes from the TaqMan Gene Expression Assays and TaqMan Gene Expression Master Mix, is performed.