Figure 2.

Broad-host-range allelic exchange vector pCM433. Plasmid map of pCM433 [GenBank:EU118176] indicating the available restriction sites in the multiple-cloning site, as well as key plasmid features: bla (encodes betalactamase; Ap^R), oriT (IncP origin of conjugal transfer), tet (encodes Tc efflux pump), cat (encodes Cm actetyltransferase), sacB (levansucrase; confers sucrose sensitivity), and ColE1 ori (high copy E. coli origin of replication). Plasmid map was generated using Clone Manager 7 (Sci Ed Central).