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DNA methylation status of NKX2-5, GATA4 and HAND1 in patients with tetralogy of fallot

Wei Sheng13, Yanyan Qian2, Huijun Wang13, Xiaojing Ma13, Ping Zhang3, Lianwei Diao5, Quan An5, Long Chen4, Duan Ma23* and Guoying Huang13*

Author Affiliations

1 Children Hospital of Fudan University, Shanghai 201102, China

2 Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 20003, China

3 Shanghai Key Laboratory of Prevention and Intervention of Birth Defects, Shanghai 201102, China

4 Department of Forensic Medicine, Fudan University, Shanghai 20003, China

5 Wuhe Chinese Medicine Hospital, Wuhe 233300, China

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BMC Medical Genomics 2013, 6:46  doi:10.1186/1755-8794-6-46

Published: 1 November 2013

Additional files

Additional file 1: Table S1:

Anagraphical characteristics of study subjects. Table S2. Primer sequences, position, product length, and CpG unit used for MassArray quantitative methylation analysis. Table S3. Primer sequences and product length for QPT-PCR analysis. Table S4. Primer sets for pyrosequencing and bisulfite sequencing PCR (BSP). Table S5. The values of ΔCt mean,ΔΔCt and RQ for NKX2-5 and HAND1 in controls and TOF subjects.

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