Cell growth, cell cycle and apoptosis in SCA28 LCLs. (A) Using the MTT assay, SCA28 LCLs displayed a reduced growth compared to controls (p < 0.001). (B) FACS analysis revealed that patients’ LCLs showed an increased number of cells in G0/G1 phase (> 15%) compared to control LCLs at any time point considered. (C) AnnexinV/PI FACS analysis showed that 41 ± 4.2% of SCA28 cells were viable, vs. 61 ± 3.6% of control cells (One-tailed Student’s t-test, p = 0.003).
Mancini et al. BMC Medical Genomics 2013 6:22 doi:10.1186/1755-8794-6-22