Table 2

The methylation profiles of 8 imprinted genes by bisulphite PCR Luminex.

Cell

Histology

H19

GTL2

ZDBF2

PEG1

LIT1

ZAC

PEG3

SNRPN


lines

BPL

COBRA

BPL

COBRA

BPL

COBRA

BPL

COBRA

BPL

COBRA

BPL

COBRA

BPL

COBRA

BPL

COBRA


CpG

(9,16)

CpG10

CpG

(4,8)

CpG8

CpG

(1,4,5)

CpG4

CpG15

CpG12

CpG

(5,17,19)

CpG16

CpG8

CpG7

CpG20

CpG21

CpG19

CpG19


NC1

N

67.5

34.6

62.2

76.8

66.4

46.2

50.4

38.5

42.7

27.4

62.3

48.4

78.8

67.4

65.3

57.3

NC2

N

55.6

42.7

67.3

82.1

67.9

72.5

54.8

46.2

53.7

37.5

65.2

69.3

65.4

48.9

78.5

67.3

NC3

N

65.8

58.3

81.2

63.5

76.3

82.1

45.3

63.9

26.4

41.6

50.2

72.1

65.8

83.2

56.3

68.2

NC4

N

58.6

72.1

76.4

80.4

80.2

67.2

37.5

58.2

35.8

54.7

47.2

52.8

45.9

58.3

65.2

74.9

OC1

S

10.32

95.4

69.8

53.2

87.3

92.4

83.1

59.2

64.3

48.3

89.5

98.3

74.4

83.2

65.4

58.4

OC2

M

16.8

19.4

61.4

70.5

95.7

97.2

48.8

62.2

62.1

48.0

20.1

10.2

92.1

89.5

29.1

49.7

OC3

S

87.4

93.2

96.3

93.4

83.7

28.6

98.3

90.4

87.4

56.9

87.3

96.5

98.5

96.4

28.4

37.4

OC4

S

42.8

56.8

97.3

93.2

99.3

98.5

0.9

10.4

13.2

17.1

100.9

98.3

48.7

47.2

28.1

24.2

OC5

S

83.4

56.4

89.2

96.6

95.3

72.8

93.2

102.4

28.4

32.6

100.3

91.3

98.8

95.6

54.3

60.4

OC6

E

78.9

72.1

94.3

82.1

38.3

18.3

83.2

68.5

34.2

56.2

85.3

78.9

57.2

97.5

40.5

53.1

OC7

C

0.4

21.3

19.8

29.3

41.7

55.5

60.5

42.0

21.2

11.3

34.5

40.1

90.7

99.0

23.5

52.8

OC8

C

0.1

6.9

6.4

10.5

91.7

87.3

81.8

88.1

35.2

29.1

49.8

53.2

102.1

95.7

21.8

33.2

OC9

S

82.3

54.3

37.4

65.2

22.4

42.8

23.6

18.5

43.7

56.8

69.5

74.9

68.5

90.2

38.2

56.3

OC10

C

83.8

67.8

78.4

65.9

27.9

32.3

63.8

58.7

42.8

60.2

89.9

93.7

59.6

43.9

38.9

71.4

OC11

S

92.4

91.6

17.1

25.0

6.0

18.0

43.2

60.1

18.2

10.0

72.5

58.2

101.4

100.0

75.5

60.5

OC12

S

54.7

53.8

23.7

18.8

48.6

74.3

68.9

58.4

48.3

93.6

98.8

67.4

39.8

73.4

57.1

51.2

OC13

S

80.3

66.2

42.3

40.1

86.0

74.2

93.4

95.2

10.5

8.8

94.4

90.5

102.1

100.0

36.0

39.5

OC14

C

98.1

87.7

67.4

40.3

99.3

90.2

99.6

95.5

12.3

1.6

34.3

41.2

92.1

100.0

38.1

51.2

OC15

C

45.2

45.0

93.0

98.2

99.7

95.3

92.1

94.8

55.0

47.5

27.5

36.4

86.1

97.2

48.7

10.1

OC16

S

36.9

44.6

90.6

92.0

93.0

92.3

100.7

100.0

13.0

6.2

28.9

35.7

93.2

100.0

22.9

17.6

OC17

C

93.8

89.8

61.8

68.3

21.8

22.2

37.9

42.5

48.4

31.8

20.6

31.1

98.3

95.5

0.0

0.2

OC18

C

34.4

46.0

94.5

98.0

95.3

98.0

60.1

73.2

35.2

29.4

17.3

35.0

87.2

100.0

22.7

11.1

OC19

C

67

30.7

47.8

62.1

87.4

85.3

4.1

10.1

11.2

0.0

100.1

100.0

84.6

92.1

30.4

31.4

OC20

M

1.6

42.6

58.1

60.3

62.4

55.2

73.4

81.4

18.3

13.4

49.2

68.2

92.8

98.5

86.7

44.8

OC21

S

8.0

24.5

80.2

87.0

96.6

90.1

77.2

81.2

4.5

8.1

100.5

97.5

107.8

98.2

16.7

16.2


Normal ovarian surface tissues

NT1 (42)

57.5

76.2

43.4

51.2

37.2

65.4

51.4

38.6

49.1

42.3

53.3

41.2

42.1

40.2

521

47.8

NT2 (48)

27.6

25.0

23.6

21.6

46.1

34.5

20.9

34.4

23.1

12.2

5.1

32.3

21.3

20.6

22.1

33.3

NT3 (40)

61.5

31.8

49.9

60.0

50.4

45.0

31.0

42.8

17.1

10.9

48.8

29.4

21.0

33.6

24.3

34.8

NT4 (38)

28.9

25.0

28.4

29.6

46.1

39.3

33.4

47.8

28.8

17.7

67.4

46.1

45.6

33.8

80.5

29.0

NT5 (55)

24.2

26.9

27.2

18.5

33.2

29.0

30.9

48.1

25.1

17.6

10.7

44.5

75.5

40.0

32.3

31.3

NT6 (45)

25.9

22.1

32.2

20.5

46.4

62.5

60.0

40.4

24.1

17.3

25.2

23.4

44.2

69.0

77.4

40.9

NT7 (41)

34.6

24.6

38.0

19.9

49.9

37.4

27.3

46.2

27.7

24.5

21.0

63.1

49.7

53.7

25.6

24.5


Letters in blue and black boldface represent LOH, LOI and MOI, respectively. The Luminex value indicates the average value of DNA methylation

Hiura et al. BMC Medical Genomics 2012 5:8   doi:10.1186/1755-8794-5-8

Open Data