Effects of PRDX3 siRNA transient transfection on the PRDX3 expression of metastatic 5-8F cells. Suppression of PRDX3 expression in the levels of mitochondria and whole cells of the metastatic 5-8F cells treated with siRNA relative to without siRNA and control siRNA treatments. The top shows the Western blotting image. The bottom shows the quantitative comparison of the digitized Western blotting image. COXIV, a marker protein of mitochondria, was used as a reference standard protein when mitochondrial protein sample was analyzed with Western blot. ACTIN that is mainly expressed in the cytoplasm was used a reference standard protein when whole-cell protein sample was analyzed with Western blot. Western blot experiments were carried out triply (n = 3).
Liu et al. BMC Medical Genomics 2012 5:62 doi:10.1186/1755-8794-5-62