The required number of of unique reads for high sensitivity across different cff-DNA concentrations. For aneuploidy detection, the required number of unique reads (y-axis) increased with decreasing cff-DNA concentration (x-axis). For a 3.5% cff-DNA concentration, 1.7 million unique reads are needed to obtain high sensitivity.
Jiang et al. BMC Medical Genomics 2012 5:57 doi:10.1186/1755-8794-5-57