Additional file 6.
Figure S1. miRNA down-regulation in bladder cell lines. LNA based antagonism were used to silence miR-27a and miR-138 in the bladder cell lines 253JBV and CLR2169. LNA knockdown molecules were reverse Transfected (n = 3) and after 48 h incubation culture media, with or without cisplatin (GI50), was added to the cells (n = 4). Expression of mature miR-27a and miR-138 was determined using real-time Q-PCR (n = 3). LF2000 designate controls using the transfection reagent alone. The 2-ΔΔCT method was used for relative quantification with miR-193b expression normalize.
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Nordentoft et al. BMC Medical Genomics 2012 5:40 doi:10.1186/1755-8794-5-40