qRT-PCR validation. qPCR was used to validate microarray results for 6 genes found to be significantly different in either Her2+ vs ER+, Her2+ vs TNBC or ER + vs TNBC comparison in microarrays data. Expression for arrays and qPCR were normalized separately over average value across absolute expression for Her2 +, ER + and TNBC groups. Error bars represent standard error of mean for the group.
Tchou et al. BMC Medical Genomics 2012 5:39 doi:10.1186/1755-8794-5-39