Table 1

List of proteins identified by mass spectrometry significantly changed between HCT116 gankyrin−/−and HCT116 cells
Spot No Protein name NCBI ID No. Abbr nme Mr (kD) pl Sequence Coverage (%) Score Change Fold
Thero Ober Thero Ober
1 Tumor protenin P53 Inducible protein 3 AAC39528 Pig3 34.4 40.2 5.9 6.0 10 115 +1.9
2 Cytochrome P450 family 1subfamily A AAX62803 CYP1A1 54.7 39.1 8.3 7.2 2 41 +3.2
3 Phosphatidylethanolamine binding protein AAB32876 PEBP1 20.9 24.9 7.4 8.0 50 347 -5.6
4 NEDD8-conjugating enzyme Ubc12 NP_003960 Ubc12 20.8 24.1 7.7 7.9 42 196 -2.0
5 26S proteasome non-ATPase regulatory subunit 10 isoform 1 NP_002805 Gankyrin 24.4 28.8 5.7 5.9 29 30 -3.3
6 S100-A9 NP_002956 S100-A9 13.2 15.0 7.4 4.2 33 149 +2.1
7 Protein kinase C inhibitor protein 1 AAH51814 YWHAZ 35.3 30.1 4.5 4.2 39 347 -9.8
8 Proliferating cell nuclear antigen 1AXC_C PCNA 28.7 38.2 4.4 3.8 44 229 -9.1
9 Tropomyosin alpha-4 chain isoform 2 NP_003281 TPM4 28.5 34.9 4.5 3.8 40 274 -3.6
10 Eukaryotic translation factor 6 isoform a NP_002203 p27BBP 26.5 30.1 4.4 3.7 15 131 -2.2
11 Nucleoplasmin-3 NP_008924 NPM3 19.3 27.0 4.4 3.7 14 182 -3.0
12 Peptidyl prolyl cis trans isomerase A NP_066953 PPIA 17.8 17.5 7.7 3.4 33 54 -5.0
13 Calcium/calmodulin-dependent protein kinase II gamma 1J7O_A CAMK2G 8.4 17.5 3.79 3.48 39 107 -2.9
14 L-lactate dehydrogenase B chain NP_002291 LDHB 36.6 45.3 5.6 5.2 8 96 +5.2
15 G protein beta subunit AAA35922 GNB2 37.3 45.1 5.7 5.2 23 218 +6.3
16 Proteasome subunit beta type-7 NP_002790 PSMB7 29.9 30.1 7.6 5.5 5 91 -26
17 Prosome beta subunit AAB31085 PSME2 25.8 29.8 5.6 5.4 28 175 -1.6
18 Proteasome subunit beta type-4 NP_002787 PSMB4 29.2 29.6 5.6 5.4 28 188 -2.3
19 Annexin A2 isoform 2 NP_004030 ANXA2 38.6 38.2 7.8 7.1 51 366 -10.5
20 Cofilin-1 NP_005498 Cofilin-1 18.5 18.9 8.2 8.4 50 347 -2.6
21 Chain K, Acetyl-Cypa:cyclosporine Comples 2X2C_K PPIA 18.0 18.0 7.2 8.1 89 1473 -3.0

Luo et al.

Luo et al. BMC Medical Genomics 2012 5:36   doi:10.1186/1755-8794-5-36

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