Open Access Highly Accessed Research article

The effects of MicroRNA transfections on global patterns of gene expression in ovarian cancer cells are functionally coordinated

Shubin W Shahab12, Lilya V Matyunina123, Christopher G Hill12, Lijuan Wang12, Roman Mezencev12, L DeEtte Walker123 and John F McDonald123*

Author Affiliations

1 School of Biology, Georgia Institute of Technology, Atlanta, GA, 30332, USA

2 Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, 30332, USA

3 Ovarian Cancer Institute, Atlanta, GA, 30342, USA

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BMC Medical Genomics 2012, 5:33  doi:10.1186/1755-8794-5-33

Published: 1 August 2012

Additional files

Additional file 1:

Figure S1. Confirmation of successful miR-128 transfection into HEY cells. Figure S2. Confirmation of EGFR down-regulation by siRNA. Figure S3. Deconvolution of DNA content histograms for Hey cells .Table S1. Differentially expressed genes in miR-7 transfected HEY cells. Table S2. Differentially expressed genes in miR-128 transfected HEY cells. Table S3. Differentially expressed genes in siRNA transfected HEY cells. Table S4. miRNA target enrichment analysis for up-regulated genes after miR-128 transfection. Table S5. Microarray expression levels of pre-transfected HEY cell miRNAs. Table S6. miRNA target enrichment analysis for up-regulated genes following miR-7 transfection. Table S7. Hub genes and their targets affected by miR-7 in HEY cells. Table S8. Hub genes and their targets affected by miR-128 in HEY cells. Table S9. Differentially expressed genes that are targeted by NF-κB or IL-1 Beta. Table S10. Direct downstream targets of Caveolin-1 and SMAD2. Table S11. GeneGo pathway maps following miR-7 transfection into HEY cells. Table S12. GeneGo pathway maps following miR-128 transfection into HEY cells. Table S13. GeneGo pathway maps for down-regulated genes following miR-7 transfection into HEY cells. Table S14. GeneGo pathway maps for down-regulated genes following miR-128 transfection into HEY cells. Table S15. GeneGo pathway maps for up-regulated genes following miR-7 transfection into HEY cells. Table S16. GeneGo pathway maps for up-regulated genes following miR-128 transfection into HEY cells.

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