Table 5

RT-qPCR results for 23 genes that were up- or down-regulated according to the microarray analysis
Detector Ct Epo-d mice Ct control mice 2-ddCt (fold change) Microarray normalized ratio
Serpinb2 26.13 12.84 9.90E-05 0.57
Nrap 24.00 12.64 3.76E-04 0.63
Chfr 19.55 14.23 2.48E-02 1.71
Plcb2 20.42 16.01 4.66E-02 1.87
Chac1 15.50 11.66 6.93E-02 0.6
Ctsd 16.82 13.12 7.68E-02 0.66
Pip4k2b 15.07 12.43 0.16 1.70
Runx1 19.07 17.73 0.39 1.88
Eno3 13.69 12.71 0.51 0.53
Mcptl 13.48 12.63 0.55 3.25
Atp1a2 12.90 12.62 0.82 0.66
Hspb7 12.67 12.78 1.08 0.58
Socs3 15.95 16.60 1.55 0.88
Ccdc130 12.38 13.31 1.90 1.74
Isg20l2 12.45 13.61 2.22 1.70
Ckm 12.26 13.58 2.47 0.52
Ints1 18.37 19.98 3.03 1.71
Zp2 10.82 12.74 3.76 0.65
Erk1 14.33 24.29 982.97 1.11
Epor 13.83 12.85 0.50 NA
Cish2 12.89 12.90 1.00 NA
Vegf 13.04 13.89 1.79 NA
Hif1a 14.08 19.84 53.82 NA

The averaged Ct values of the triplicate analysis are quoted for EPO-deficient and control mice. The Ct values were normalized against Cish2 expression because the latter was not affected by the EPO deficiency. The relative expression of each gene in EPO-deficient mice was calculated by comparison with the corresponding gene expression in control mice. There was a statistically significant correlation (Pearson coefficient: 0.87; pā€‰<ā€‰0.01) between the expression values for 19 genes according to the RT-qPCR assays and the expression values according to the microarray analysis.

Mille-Hamard et al.

Mille-Hamard et al. BMC Medical Genomics 2012 5:29   doi:10.1186/1755-8794-5-29

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