Table 3

Study population and biologic samples1
Parameter Healthy nonsmokers Healthy smokers
n 60 72
Gender (male/female) 38/22 51/21
Age 41 ± 12 43 ± 8
Race (B/W/O)2 27/23/10 45/16/11
Smoking history (pack-yr) 0 27 ± 16
Urine nicotine (ng/ml) negative 1098 ± 1312
Urine cotinine (ng/ml) negative 1228 ± 939
Pulmonary function parameters4
FVC (% predicted) 106 ± 13 110 ± 14
FEV1 (% predicted) 106 ± 14 110 ± 15
FEV1/FVC (% observed) 82 ± 6 81 ± 4
TLC (% predicted) 99 ± 13 100 ± 12
DLCO (% predicted) 98 ± 14 94 ± 11
Epithelial cells4
Number recovered x106 6.5 ± 2.9 7.4 ± 3.3
% epithelial cells5 99.1 ± 1.2 99.0 ± 1.3
% inflammatory cells 0.9 ± 1.2 1.0 ± 1.3
Differential cell count6
Ciliated (%) 72.0 ± 8.9 64.0 ± 12
Secretory (%) 6.7 ± 3.7 8.4 ± 4.4
Basal (%) 12.6 ± 6.6 14.5 ± 8.0
Intermediate (%) 7.8 ± 3.4 12.2 ± 6.8

1 Data are presented as mean ± standard deviation.

2 B = Black, W = White, O = Other.

3 Pulmonary function testing parameters are given as % of predicted value with the exception of FEV1/FVC, which is reported as % observed; FVC - forced vital capacity; FEV1 - forced expiratory volume in 1 sec; TLC - total lung capacity; DLCO - diffusing capacity.

4 To quantify the percentage of epithelial and inflammatory cells and the proportions of ciliated, basal, secretory, and intermediate epithelial cells, aliquots of 2x104 cells were prepared by centrifugation and stained with DiffQuik.

5 Of total cells recovered.

6 Of total epithelial cells recovered.

Wang et al.

Wang et al. BMC Medical Genomics 2012 5:21   doi:10.1186/1755-8794-5-21

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