Figure 6 .
Proposed gene network of the MUC5AC-core genes. From the MUC5AC-core gene list, genes with at least one literature supported connection to other MUC5AC-core genes were selected (see Methods for detail). The connection denotes that the expression level of one gene is affected by the other gene; the data is derived from literature with in vitro gene overexpression/activation/knockdown experiments or transgenic mice data (see Additional file 1: Table S5 for references). Protein to protein interactions are not included. Genes in the brown solid circle include, transcription factors (SPDEF, SOX2, KLF4) and the Wnt, Notch, MAPK pathways, which can affect MUC5AC-producing cell differentiation. A red dashed circle was used to emphasize their important roles. Genes in green solid circle represent transcription factors in the MUC5AC-core gene list with literature supported potential role in MUC5AC-producing cells. Genes in green rectangles represent non-transcription factors in the MUC5AC-core gene list. Arrows indicate upstream gene can regulate downstream gene expression. Dashed arrow, based on mouse data , KLF4 can affect mucus-producing cell differentiation, but there is no direct evidence showing KLF4 can affect MUC5AC expression. The solid line linked to pathway indicates that MAPK13, KRAS and RPS6KA3 belong to “MAPK pathway”; HES1 and TSTA3 belong to “Notch pathway”; LRRFIP2 belong to “Wnt pathway”. The “T” like symbol indicates a potential blocking effect.
Wang et al. BMC Medical Genomics 2012 5:21 doi:10.1186/1755-8794-5-21