Workflow for the PCR enrichment approach. Amplicons are equimolar pooled per patient, continuously the A and B (454) adapters with MID are ligated to each pool of amplicons. All the patients, each tagged with a unique MID, can be pooled before proceeding to emulsion PCR.
De Keulenaer et al. BMC Medical Genomics 2012 5:17 doi:10.1186/1755-8794-5-17