Figure 3.

Workflow for the PCR enrichment approach. Amplicons are equimolar pooled per patient, continuously the A and B (454) adapters with MID are ligated to each pool of amplicons. All the patients, each tagged with a unique MID, can be pooled before proceeding to emulsion PCR.

De Keulenaer et al. BMC Medical Genomics 2012 5:17   doi:10.1186/1755-8794-5-17
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