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Open Access Research article

Comparative analysis of the human hepatic and adipose tissue transcriptomes during LPS-induced inflammation leads to the identification of differential biological pathways and candidate biomarkers

Ewa Szalowska15*, Martijn Dijkstra1, Marieke GL Elferink2, Desiree Weening1, Marcel de Vries1, Marcel Bruinenberg3, Annemieke Hoek4, Han Roelofsen1, Geny MM Groothuis2 and Roel J Vonk1

Author Affiliations

1 Centre for Medical Biomics, University Medical Centre Groningen (UMCG), University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands

2 Division of Pharmacokinetics, Toxicology and Targeting; Department of Pharmacy University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands

3 Department of Genetics, University Medical Centre Groningen (UMCG), P.O. Box 30001, 9700 RB Groningen, The Netherlands

4 Department of Obstetrics and Gynecology, University Medical Centre Groningen (UMCG), PO Box 30.001, 9700 RB Groningen, The Netherlands

5 Wageningen University and Research Centre/RIKILT, Cluster of Toxicology and Effect Analysis, Postbus 230, 6700AE Wageningen, The Netherlands

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BMC Medical Genomics 2011, 4:71  doi:10.1186/1755-8794-4-71

Published: 6 October 2011

Abstract

Background

Insulin resistance (IR) is accompanied by chronic low grade systemic inflammation, obesity, and deregulation of total body energy homeostasis. We induced inflammation in adipose and liver tissues in vitro in order to mimic inflammation in vivo with the aim to identify tissue-specific processes implicated in IR and to find biomarkers indicative for tissue-specific IR.

Methods

Human adipose and liver tissues were cultured in the absence or presence of LPS and DNA Microarray Technology was applied for their transcriptome analysis. Gene Ontology (GO), gene functional analysis, and prediction of genes encoding for secretome were performed using publicly available bioinformatics tools (DAVID, STRING, SecretomeP). The transcriptome data were validated by proteomics analysis of the inflamed adipose tissue secretome.

Results

LPS treatment significantly affected 667 and 483 genes in adipose and liver tissues respectively. The GO analysis revealed that during inflammation adipose tissue, compared to liver tissue, had more significantly upregulated genes, GO terms, and functional clusters related to inflammation and angiogenesis. The secretome prediction led to identification of 399 and 236 genes in adipose and liver tissue respectively. The secretomes of both tissues shared 66 genes and the remaining genes were the differential candidate biomarkers indicative for inflamed adipose or liver tissue. The transcriptome data of the inflamed adipose tissue secretome showed excellent correlation with the proteomics data.

Conclusions

The higher number of altered proinflammatory genes, GO processes, and genes encoding for secretome during inflammation in adipose tissue compared to liver tissue, suggests that adipose tissue is the major organ contributing to the development of systemic inflammation observed in IR. The identified tissue-specific functional clusters and biomarkers might be used in a strategy for the development of tissue-targeted treatment of insulin resistance in patients.