Comparison of different tumor biopsies for targeted re-sequencing approaches. (A) Normalized coverage-distribution plot for two foci of each of three tumor tissues. The mean coverage per exon was divided by the overall mean coverage of all exons and plotted as normalized coverage (x-axis). The fraction of bait-covered exons in the genome achieving coverages equal or lower than the overall mean coverage is indicated on the y-axis. (B) Exonwise coverage comparison of two foci of each of three tumor tissues. A coefficient of variation is calculated for each comparison and plotted by the smallest coverage of each exon-exon comparison. (C) Comparison of SNVs detected in two foci of each of three tumors. Discordant SNVs are those detected in focus A but not focus B and vice versa. (D) Comparison of InDels detected in two foci of each of three tumors. Discordant InDels are those detected in focus A but not focus B and vice versa.
Kerick et al. BMC Medical Genomics 2011 4:68 doi:10.1186/1755-8794-4-68