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Targeted high throughput sequencing in clinical cancer Settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity

Martin Kerick1, Melanie Isau12, Bernd Timmermann1, Holger Sültmann3, Ralf Herwig1, Sylvia Krobitsch1, Georg Schaefer45, Irmgard Verdorfer56, Georg Bartsch4, Helmut Klocker4, Hans Lehrach1 and Michal R Schweiger1*

Author Affiliations

1 Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195 Berlin, Germany

2 Free University Berlin, Department of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195 Berlin, Germany

3 Unit Cancer Genome Research, DKFZ German Cancer Research Center and National Center for Tumor Diseases, Im Neuenheimer Feld 460, 69120 Heidelberg, Germany

4 Innsbruck Medical University, Department of Urology, Anichstr. 35, A 6020 Innsbruck, Austria

5 Innsbruck Medical University, Department of Pathology, Muellerstr. 40, A-6020 Innsbruck, Austria

6 Innsbruck Medical University, Department for Medical Genetics, Molecular and Clinical Pharmacology, Division of Human Genetics, Schöpfstraße 4, 6020 Innsbruck, Austria

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BMC Medical Genomics 2011, 4:68  doi:10.1186/1755-8794-4-68

Published: 29 September 2011

Additional files

Additional file 1:

Supplementary Methods, Figure Legends S1-S6, Table Legends S1-S3. Figures S1-S6

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Additional file 2:

Tables S1-S3.

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