Open Access Research article

Gene expression during normal and FSHD myogenesis

Koji Tsumagari1, Shao-Chi Chang1, Michelle Lacey23, Carl Baribault23, Sridar V Chittur4, Janet Sowden5, Rabi Tawil5, Gregory E Crawford6 and Melanie Ehrlich13*

Author Affiliations

1 Human Genetics Program, Tulane Medical School, New Orleans, LA, USA

2 Department of Mathematics, Tulane University, New Orleans, LA, USA

3 Tulane Cancer Center, New Orleans, LA, USA

4 Biomedical Sciences, University at Albany-SUNY, Albany, NY, USA

5 Department of Neurology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA

6 Institute for Genome Sciences & Policy, Duke University, Durham, NC, USA

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BMC Medical Genomics 2011, 4:67  doi:10.1186/1755-8794-4-67

Published: 27 September 2011

Additional files

Additional file 1:

Table S1. Description of myoblast and myotube samples for microarray analysis

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Additional file 2:

Table S2. Descriptions of non-muscle cell types for expression profiling

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Additional file 3:

Table S3. Description of myoblast and myotube samples for qRT-PCR

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Additional file 4:

Additional Methods: Generation, propagation, and differentiation of myoblast cultures from human skeletal muscle.

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Additional file 5:

Table S4. qPCR primers and full names of genes tested.

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Additional file 6:

Figure S1. FSHD-downregulated genes: relationship between gene expression in FSHD myotubes vs. non-muscle cell types to that in control myotubes vs. non-muscle cell types.

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Additional file 7:

Figure S2. FSHD-upregulated genes: relationship between gene expression in FSHD myotubes vs. non-muscle cell types to that in control myotubes vs. non-muscle cell types.

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Additional file 8:

Table S5. Some pathways and functional terms overrepresented among FSHD-dysregulated genes.

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Additional file 9:

Table S6. Most of the pro- or anti-apoptotic genes that were dysregulated in FSHD vs. control myotubes were apparently up- or downregulated in FSHD myotubes because of dampening of their normal myogenesis-associated changes in expression.

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