Epigenetic modifications of histone H3 and RelB expression: histone H3K9-K14 acetylation in the RELB promoter (using ChIP and Q-PCR). Chromatin was immunopurified by anti-3meH3K9 or anti-acetyl-H3 immunoprecipitations (ChIP) and the levels of 3metH3K9 and acetyl-H3 in the three promoter regions of RELB were established by Q-PCR SYBR Green. These levels were expressed as the percentage of the input chromatin fraction defined in Method section. Ns: non significant; *for resistant male samples the values for the level of acetyl-H3 were too low to allow the calculation of the percentages. The sequences of the primers used for amplifying the three indicated RELB promoter regions were the same as described for histone H3K9 trimethylation (Figure 1).
Marteau et al. BMC Medical Genomics 2010 3:53 doi:10.1186/1755-8794-3-53