Epigenetic modifications of histone H3 and RelB expression: histone H3K9 trimethylation in the RELB promoter (using ChIP and Q-PCR). Chromatin was immunopurified by anti-3meH3K9 immunoprecipitation (ChIP) and the levels of 3metH3K9 in the three promoter regions of RELB were established by Q-PCR SYBR Green. These levels were expressed as the percentage of the input chromatin fraction defined in Material and Method section. Ns: non significant. The sequences of the primers used for amplifying the three indicated RELB promoter regions are: 74 bp amplicon: forward primer 5' CTGGTGATAGGGATGAGC 3' and reverse primer 5' CGAATGGCAGCAGTGTA 3'; 121 bp amplicon: forward primer 5' GGGTTACAACAACGCACAA 3' and reverse primer 5' CCTCCAAGGTCTCGCTAC 3'; 58 bp amplicon: forward primer 5' TGCTCAATGGGTAAGGC 3' and reverse primer 5' TGCTCTGGACGAGACAAC3'.
Marteau et al. BMC Medical Genomics 2010 3:53 doi:10.1186/1755-8794-3-53