Table 2

Production of chemokines by lung lymphocytes ex vivo after immunization with Ad85A i.d. or i.n..

Absorbance at 450nm (± SD)


Chemokine

i.d.

i.n.


CCL2

0.89 (± 0.427)

1.368 (± 0.131)


CCL3

0.334 (± 0.066)

0.745 (± 0.008)


CCL4

0.278 (± 0.011)

0.760 (± 0.028)


CCL5

0.386 (± 0.098)

1.470 (± 0.211)


CXCL9

0.259 (± 0.059)

0.566 (± 0.034)


Conc (pg/ml)


CXCL16

3.34 (± 0.339)

6.095 (± 0.977)


Lung lymphocytes were isolated from mice immunized 3 weeks previously and cultured in RPMI+10% FCS for 6 hours at 37°C without antigen stimulation. The supernatants were assayed by ELISA for the indicated chemokines. The table shows the mean absorbance at 450 nm (± standard deviation (SD)) of 6 mice per group. For CXCL16, the mean concentration of CXCL16 (pg/ml, ± SD) of 6 mice per group is shown.

Lee et al. BMC Medical Genomics 2010 3:46   doi:10.1186/1755-8794-3-46

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