Additional file 2.

Supplementary Figure S2. We checked the technical reproducibility of Agilent microarray results using the 3 patient samples analyzed here. We investigated the robustness of gene expression profiles in dependence of: 1. the experimenter (E); or 2. repeating the labelling (L); or 3. repeating the hybridization (H); or 4. using different washing methods (W). These types of technical replicates where highly correlated and clustered together. Supplementary Figure 2 shows pairwise correlations between all samples of patients. The elements of the matrix in the visualization are colored by Pearson's correlation coefficient values with deeper colors indicating higher positive (blue) correlations. The heatmap is flanked by clustering dendrograms showing the similarity between samples in a hierarchical approach.

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Opitz et al. BMC Medical Genomics 2010 3:36   doi:10.1186/1755-8794-3-36