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Open Access Research article

Astrocytes derived from trisomic human embryonic stem cells express markers of astrocytic cancer cells and premalignant stem-like progenitors

Sailesh Gopalakrishna-Pillai and Linda E Iverson*

Author Affiliations

Stem Cell Biology, Beckman Research Institute of the City of Hope, 1450 E. Duarte Road, Duarte CA, 91010, USA

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BMC Medical Genomics 2010, 3:12  doi:10.1186/1755-8794-3-12

Published: 27 April 2010

Additional files

Additional file 1:

Table S1: RT-PCR Primer Sequences. Sequences of forward and reverse primers used for qRT-PCR validation and list of all transcripts where changes in expression levels, first detected by microarray analysis, were subsequently validated by qRT-PCR analysis.

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Additional file 2:

Table S2: Differentially expressed gene transcripts in GD v. C cell populations. Excel spreadsheet of all differentially expressed genes in trisomic BG01V APCs (samples G) and CCF-STTG1 astrocytoma cells (samples D) relative to H9 APCs (samples C) and referred to in the text as the GDvC comparison. All data filtered using a p value of < 0.02.

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Additional file 3:

Figure S1: RT-PCR analysis using Human Cancer Pathway Finder PCR Arrays. The four panels on the left show quantitative changes in expression levels of all cancer-associated gene transcripts included in the Cancer Super Arrays. The heat map displaying relative over expression (red) or under expression (green) of these cancer-associated gene transcripts in samples C (diploid H9 APCs) and samples G (trisomic BG01V APCs) is shown in the right panel.

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Additional file 4:

Table S3: Differentially expressed gene transcripts in GN v. C cell populations. Excel spreadsheet of all differentially expressed genes in BG01V APCs (samples G) and glioblastoma patient samples (samples N) relative to H9 APCs (samples C) and referred to in the text as the GNvC comparison. All data filtered using a p value of < 0.02.

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Additional file 5:

Table S4: Differentially expressed gene transcripts in astrocytic cancer cells (GND v. C). Excel spreadsheet of all genes exhibiting differential expression patterns in BG01V APCs (samples G), glioblastoma patient samples (samples N) and CCF-STTG1 astrocytoma cells (samples D) relative to H9 APCs (samples C). The list contains the 499 genes identified (indicated in Figure 6) at the intersection of GDvC (Additional file 2, Table S2) and GNvC (Additional file 4, Table S3) and referred to in the text as the GNDvC comparison. All data filtered using a p value of < 0.02.

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Additional file 6:

Table S5: Differentially expressed gene transcripts in premalignant astrocytic stem-like/progenitor cells (GN v. CD). Excel spreadsheet of the 311 differentially expressed genes (indicated in Figure 7) in BG01V APCs (samples G) and glioblastoma patient samples (samples N) relative to CCF-STTG1 astrocytoma cells (samples D) and H9 APCs (samples C) and referred to in the text as the GNvCD comparison. All data filtered using a p value of < 0.02.

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Additional file 7:

Figure S2: Transcript expression profile of genes associated with glioblastomas and/or genes that map to trisomic chromosomes. Dot plots of relative expression levels of several gene transcripts located on trisomic chromosomes (12 or 17) and several genes associated with brain tumors are shown for glioblastoma patient samples (purple, N), trisomic BG01V APCs (green, G), CCF-STTG1 astrocytoma cells (blue, D) and diploid H9 APCs (red, C). Transcripts shown are EGFR (panel A), PTEN (panel B), AKT1 (panel C), IL13RA2 (panel D), NRG1 (panel E), PDGFB (panel F), PDGFRA (panel G), PDGFRB (panel H), TP53 (panel I), MDM2 (panel J), NF1 (panel K), RB1 (panel L), STAT3 (panel M), ERBB2 (panel N), RARA (panel O) and RARG (panel P).

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