KSHV infection upregulates the expression of lymphatic markers but K13 fails to do so. HUVECs were infected with KSHV for the indicated time intervals and the expression of lymphatic markers LYVE-1, VEGFR-3 and PROX-1 was determined by qRT-PCR analysis. In parallel, the expression of the lymphatic markers was determined in HUVECs-K13-ERTAMand -vector cells that had been left untreated or treated with 4OHT (50 nM) for 48 h. The experiment was performed as described in the Materials and Methods section.
Punj et al. BMC Medical Genomics 2009 2:50 doi:10.1186/1755-8794-2-50