Validation of gene expression array data by qRT-PCR and western blotting. (a). Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ERTAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b). Linear regression analysis shows excellent correlation between the qRT-PCR and microarray data with a coefficient of variation (R2) of 0.845. (c). Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ERTAM cells with 4OHT. Tubulin blot shows equal protein loading.
Punj et al. BMC Medical Genomics 2009 2:50 doi:10.1186/1755-8794-2-50