Table 5

Diagnosis effect on the candidate control genes.

ΔΔCt Method

Comparative Ct Method


Normalized to TRAP1

Normalized to Optimal Set of 4 Genes*


Gene

Fold Change (MS/H)

Fold Change (MS/H)

p-value(Diagnosis)


FPGS

1.08

1.00

0.964

ACTb

1.08

ND

0.788

GINS2

1.21

1.11

0.691

HMBS

1.12

1.06

0.581

B2M

-1.04

-1.10

0.575

FARP1

1.34

1.16

0.559

A4GALT

-1.01

ND

0.416

TRAP1

**

-1.08

0.342

GAPDH

-1.45

-1.61

0.198

DECR1

-1.09

-1.11

0.161

PPIB

1.32

1.22

0.155

PEX16

1.32

1.23

0.073

MAPRE2

1.42

1.30

0.072

CRY2

1.58

1.45

0.065

CSNK1G2

1.76

1.63

0.026


Expression of the candidate reference genes in whole blood of multiple sclerosis (MS) subjects is compared to age and gender matched control subjects using the ΔΔCt Method and the Comparative Ct Method. Genes are arranged in the order of decreasing p-value for diagnosis.

*ANOVA: μ + Diagnosis + Plate + (Diagnosis × Plate) + ε

**TRAP1 fold change not calculated since that gene was used as a normalizer.

Stamova et al. BMC Medical Genomics 2009 2:49   doi:10.1186/1755-8794-2-49

Open Data